Author name: Richard Scheltema

Flexible domains on membrane proteins and how to study them – combining cryo-EM, limited proteolysis-coupled and crosslinking mass spectrometry Diane C.A. Barret1 *, Dina Schuster1, 2, 3 *, Matthew J. Rodrigues1, Alexander Leitner2, Paola Picotti2, Gebhard F.X. Schertler1, U. Benjamin Kaupp4, 5, Volodymyr M. Korkhov1, 3 and Jacopo Marino1 1 Laboratory of Biomolecular Research, Paul

Thrombin activation of the factor XI dimer is a multi-staged process for each subunit Awital Bar Barroeta1, Pascal Albanese2,3, J. Arnoud Marquart1, Joost C.M. Meijers1,4, Richard A. Scheltema2,3 1 Department of Molecular Hematology, Sanquin, Amsterdam, the Netherlands2 Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University,

Quantitative crosslinking and mass spectrometry indicates kinetochore complex stabilization Goetz Hagemann1, Victor Solis-Mezarino1, Sylvia Singh1, Mia Potocnjak1, Chandni Kumar1, Benjamin Neuditschko2 and Franz Herzog1,2,* 1 Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany2 Institute Krems Bioanalytics, IMC University of Applied Scienes Krems, 3500 Krems, Austria Introduction: Distance restraints derived from the mass

Direct single molecule imaging on a modified Q Exactive UHMR with electron holography capability Albert Konijnenberg1, Josh Gilbert2, Kyle Fort3, Alexander Makarov3, Alan Bahm2 1 Thermo Fisher Scientific, Achtseweg Noord 5, 5651 GG Eindhoven, the Netherlands2 Thermo Fisher Scientific, 5350 NE Dawson Creek Drive, Hillsboro, OR97124, USA3 Thermo Fisher Scientific, Hannah-Kunath-StraBe 11, 28199 Bremen, Germany

Exploring cellular organization in native cell extracts with high-resolution cryo-EM Panagiotis L. Kastritis1,2 1 Institute of Biochemistry and Biotechnology; Biozentrum; and Interdisciplinary Research Center HALOmem, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany;2 Institute of Chemical Biology, National Hellenic Research Foundation, Athens, Greece Advances in electron microscopy have provided unprecedented access to the structural characterization of large,

A bipartite GINS binding mode of TopBP1 activates the replicative helicase MCM Matthew Day*2, Bilal Tetik*1, Milena Parlak*1, Yasser Almeida-Hernández3, Elsa Sanchez-Garcia3, Farnusch Kaschani4, Markus Kaiser4, Markus Räschle5, Heike Siegert1, Anika Marko1, Isabel A. Barker2, Laurence H. Pearl2+, Antony W. Oliver2+, Dominik Boos1+ 1 Molecular Genetics II, Vertebrate DNA Replication, Centre of Medical Biotechnology, University